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. 2022 Feb 8;10(2):403. doi: 10.3390/biomedicines10020403

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Immunofluorescence analysis of Vinculin expression. Left panel shows the images of hDPSCs (CTRL), hDPSCs+5-Aza (5-Aza), hDPSCs+EVs (EVs), and hDPSCs+5-Aza+EVs (5-Aza+EVs) cultured without DDP. Right panel shows the images of hDPSCs (CTRL), hDPSCs+5-Aza (5-Aza), hDPSCs+EVs (EVs), and hDPSCs+5-Aza+EVs (5-Aza+EVs) cultured without DDP. Red fluorescence: ALP marker; green fluorescence: cytoskeleton actin; blue fluorescence: cell nuclei using TOPRO; merged image: overlap of all above mentioned channels. Representative images are shown. Scale bar: 20 µm.