Fig. 5.

MIR210HG is induced by IGF2BP1 mediated m6A modification. A MIR210HG genome scheme of m6A modification sites. Four m6A sites were indicated. B and C MeRIP assays were performed to identify variation in m6A modification and IGF2BP1 enrichments in MIR210HG after overexpressing MIR210HG in breast cancer cells. D q-PCR analysis of ectopic expression of IGF2BP1 achieved by pcDNA3.1-IGF2BP1 vector transfection in MCF-7 cells. E q-PCR analysis of IGF2BP1 silencing achieved by IGF2BP1 siRNA pools in MCF-7 cells. F and G the degradation of MIR210HG transcript was measured by q-PCR at different time points with indicated treatments. H q-PCR analysis of ELAVL1, MATR3 and PABPC1 in the indicated cell lines. I The degradation of MIR210HG transcript was measured by q-PCR at different time points after silencing ELAVL1. J The degradation of MIR210HG transcript was measured by q-PCR at different time points with indicated treatment. K Co-IP-western blot analysis of ELAVL1 by precipitation with IGF2BP1 antibody in MDA-MB-231 cell. *p < 0.05, **p < 0.01, ***p < 0.001