Fig. 5.

Development of skin lesions and systemic inflammation in old Mcpip1^EKO mice. a Macroscopic appearance of 6-month-old mice. b Kaplan-Meier plots for skin inflammation incidences (n = 22). c Body weights of 6- to 8-month-old mice (n = 12). d Spleen and lymph node images of 6-month-old mice. e Spleen weights of 6- to 8-month-old mice (n = 5). f Flow cytometric analysis of 6- to 8-month-old mouse splenic CD45+ cells (n = 4). g H&E staining of control and unaffected Mcpip1^EKO back skin and Mcpip1^EKO skin lesions. h Quantification of the epidermal thickness (n = 6). Mcpip1^EKO lesional skin was compared to control skin. i Quantification of hypodermis in 6-month-old mice (n = 4). j Keratin 14 and keratin 10; k PCNA and keratin 6 immunofluorescence staining. Data represent the mean ± SEM; epi, epidermis; der, dermis. The dashed line indicates the basal membrane. Scale bar, 100 μm. *P < 0.05, **P < 0.01, ***P < 0.001 by unpaired t test or one-way ANOVA