Fig. 3.

CircRbms1 directly sponged miR-742-3p. A The fragments of circRbms1-WT and circRbms1-MUT. B The transfection efficiency of miR-742-3p mimic (50 nM) in H9c2 cells was confirmed by detecting miR-742-3p expression using qRT-PCR. Dual-luciferase reporter assay (C), RNA pull-down assay (D), and RIP assay (E) were used to verify the interactions between circRbms1 and miR-742-3p. F In H9c2 cells transfected with si-NC (50 nM), si-circRbms1 (50 nM), vector (4.0 µg), or circRbms1 (4.0 µg), the expression of miR-742-3p was examined by qRT-PCR. G MiR-742-3p expression in the heart tissues of sham mice (n = 6) and MI mice (n = 6) was assessed using qRT-PCR. H The expression of miR-742-3p in hypoxia-treated and untreated H9c2 cells was measured by qRT-PCR. All experiments were repeated three times. ***P < 0.001