Fig. 7.

Effects of miR-742-3p and FOXO1 on hypoxia-induced H9c2 cell injury. A H9c2 cells were transfected with pcDNA3.1 and pcDNA3.1-FOXO1, and the protein expression of FOXO1 was detected by WB analysis. B–F H9c2 cells were transfected with miR-NC (50 nM), miR-742-3p (50 nM), miR-742-3p (50 nM) + pcDNA3.1 (4.0 µg), or miR-742-3p (50 nM) + pcDNA3.1-FOXO1 (4.0 µg), and then treated with hypoxia. Untreated H9c2 cells were used as control. Cell viability, migrated and invaded cell numbers, and cell apoptosis rate were determined by CCK8 assay (B), transwell assay (C, D), and flow cytometry (E). F WB analysis was employed to examine the protein levels of Bcl-2, Bax and Cleaved-caspase 3. All experiments were repeated three times. **P < 0.01, ***P < 0.001