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. 2022 Mar 28;23:85. doi: 10.1186/s13059-022-02652-8

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Runtime and closest homolog identification accuracy for SHOOT, BLAST, and DIAMOND. A Mean runtime and 95% confidence interval for 1000 searches of randomly sampled sequences against the same database of 984,137 protein sequences from 78 species. B Accuracy at identifying the closest related database gene to a randomly selected query sequence. C Mean average precision at k (MAP@k)