Fig. 4.

SeNPs modulated ROS accumulation and antioxidant enzymes related genes in rapeseed seedlings. a Nitro blue tetrazolium (NBT) and 3, 3-diaminobenzidine (DAB) staining accumulation under different doses of SeNPs and Se (IV) (0, 50, 100 and 150 µmol/L) on Yangyou 9 leaves; b roots stained with DAB; c roots stained with NBT. d–h The transcription levels of d superoxidase (SOD); e peroxidase (POD); f catalase (CAT); g ascorbate peroxidase (APX) and h glutathione reductase (GR) related genes in rapeseed shoot and root tissues. Bars represent ± SE of three replicates. The different letters indicate significant differences at P < 0.05 using Duncan's multiple range tests