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Sequences of the primers and probes used.
| Gene | Forward primer sequence (5’-3’) | Reverse primer sequence (5’-3’) | Probe oligo sequence |
|---|---|---|---|
| Methylight | |||
| IL-6 | TTATATTATATAGACGGATTATAGTGTACGG | TTTTAATACTCTCCTATCTTAAACAACGTA | AAAACGAAACCACTACTCCCAACTCCGCAA |
| ACTB | TGGTGATGGAGGAGGTTTAGTAAGT | AACCAATAAAACCTACTCCTCCCTTAAA | ACCACCACCCAACACACAATAACAAACACA |
| RT–qPCR | |||
| IL-6 | ATGCAATAACCACCCCTGAC | GAGGTGCCCATGCTACATTT | |
| β-actin | ATGGGTCAGAAGGATTCCTATGTG | CTTCATGAGGTAGTCAGTCAGGTC |
