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. Author manuscript; available in PMC: 2022 Oct 1.
Published in final edited form as: Nat Neurosci. 2021 Aug 9;24(10):1402–1413. doi: 10.1038/s41593-021-00903-8

Extended Data Fig. 9. SNR→LPB neurons have very few collaterals to NAcLat-projecting VTA DA neurons.

Extended Data Fig. 9.

(a) Schematic showing injection of fluorogold (FG) into the LPB and AAVs encoding the cellular receptor for subgroup A avian leukosis viruses (TVA) and rabies virus glycoprotein (RG) in the VTA of DAT-Cre mice. In the same animals, EnvA-pseudotyped, glycoprotein-deficient rabies virus expressing GFP (EnvA-RV-GFP) was targeted to the NAcLat. (b) Representative example of coronal section of the ventral midbrain showing retrogradely-labeled cells in the lateral SNR (i.e., LPB-projecting, FG-positive, red). GFP-positive cells (green) make monosynaptic connections onto VTA DA neurons projecting to NAcLat and are mainly located in the substantia nigra pars compacta (SNc), ventral SNR (vSNR) and lateral VTA, but do not overlap with the lateral SNR (lSNR; scale bar 100 μm). (c) Pie chart showing proportion of analyzed cells (n = 4035 cells from n = 3 mice) in the ventral midbrain that express GFP (green, 19.7%) or are labeled by FG (red, 79.4%) or contain both GFP and FG (yellow, 0.9%).