TABLE 2.
The expression of CD4, CD26, and CXCR4 by different cell lines
| Cell line | Cell surface expressiona
|
||
|---|---|---|---|
| CD4 | CXCR4 | CD26 | |
| CEM | 99.6 ± 0.8 (164 ± 22) | 99.1 ± 0.8 (225 ± 65) | 1.1 ± 0.2 (5.2 ± 0.2) |
| SUP-T1 | 99 ± 1 (228 ± 15) | 99.8 ± 0.1 (425 ± 88) | 2.8 ± 0.4 (4.9 ± 0.5) |
| MT-4 | 98.6 ± 0.6 (138 ± 22) | 32 ± 12 (18 ± 6) | 1.5 ± 0.2 (3.7 ± 0.1) |
| PBMC (CD4+ T-cells)b | 100 (133 ± 42)b | 63 ± 14 (62 ± 22) | 52 ± 22 (19 ± 6) |
a
The expression of CD4, CD26, and CXCR4 was evaluated by FACS analysis as described in Materials and Methods. Results show the percentage of positive cells followed by the mean fluorescence intensity of the cell surface staining. Data represent the means ± the standard deviations of three independent experiments.
b
CD4+ T cells were gated for analysis of expression of CXCR4 and CD26.