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. 2022 Mar 11;13:825834. doi: 10.3389/fimmu.2022.825834

Figure 2.

Figure 2

Ischemic PC does not affect spleen contraction induced by MCAo, while it elevates expression of the M2 marker Ym1 in spleen but not in circulating leukocytes. (A) Spleen weight and protein expression levels of Ym1 in spleen (B) and in circulating leukocytes (D) of mice subjected to SHAM surgery, ischemic PC (15 min MCAo followed by 72h of reperfusion), 1h MCAo followed by 24h of reperfusion (MCAo) or PC+MCAo. (C) Representative immunofluorescence images showing Ym1 expression (green fluorescence) in CD11b immunopositive myeloid cells and Ly-6G immunopositive neutrophils (red fluorescence) in the spleen parenchyma of mice undergone 1h MCAo preceded (72h before) by sham surgery (MCAo) or by ischemic PC (PC+MCAo). Scale bars = 50 μm. Data were analysed by one-way ANOVA followed by Tukey’s post-hoc test [(A) #P = 0.0083, ##P = 0.0057 and ###P = 0.0002 vs SHAM, n = 10-13 mice per experimental group; (B) **P = 0.0046 vs SHAM, ***P = 0.0001 vs SHAM and P = 0.0037 vs MCAo, n = 8-13 mice per experimental group; (D) §§P = 0.0098 vs SHAM, P = 0.0013 vs PC and P = 0.0051 vs PC+MCAo, n = 5-6 mice per experimental group].