TABLE 5.

Influence of culture conditions on MIC^a

Culture condition	MIC (μg/ml)
	C. albicans ATCC 90028b	C. glabrata ATCC 90030b	A. fumigatus TIMM0063c
Kind of medium
RPMI 1640	0.0156	0.0156	0.0039
YNBD	0.0625	0.0156	≤0.002
SD	0.0625	0.0313	≤0.002
PYG	0.0313	0.0313	≤0.002
pH of medium
5	0.0313	0.0156	0.0078
6	0.0156	0.0156	0.0039
7	0.0078	0.0156	0.0039
8	0.0313	0.0078	0.25
Inoculum size (cells/ml)
102	0.0156	0.0313	≤0.002
103	0.0156	0.0313	≤0.002
104	0.0156	0.0625	0.0156
105	0.0156	0.0625	0.0156
Addition of HSA
0%	0.0156	0.0156	0.0078
0.04%	0.0156	0.0625	0.0078
0.4%	0.25	0.25	0.0313
4.0%	2	4	0.25

^aBroth microdilution testing was used. The MICs for Candida species were determined as the minimum concentration resulting in at least 80% inhibition of growth of Candida species measured as a decrease in turbidity compared with the turbidities of the growth controls. The MICs for A. fumigatus were determined as the minimum concentration resulting in a prominent decrease in turbidity compared with the turbidity of the growth control. 

^bIncubation was for 48 h at 35°C. 

^cIncubation was for 72 h at 35°C.