Table. Summary of results of experimental inoculation of raccoons with the agent of CWD from white-tailed deer or vole-passaged CWD isolates* .
| Raccoon no. |
Inoculum |
Incubation time, mpi |
Clinical signs |
EIA OD |
Spongiform change |
Immunohistochemistry |
||||
|---|---|---|---|---|---|---|---|---|---|---|
| Brain |
Retina |
Pituitary |
ENS |
LRS |
||||||
| 1 | WTD CWD (CWDWTD) |
21 | + | 4.000 | + | + | + | + | – | – |
| 2 | 22 | + | 4.000 | + | + | + | – | – | – | |
| 3 | 27 | + | 3.244 | + | + | + | + | + | – | |
| 4 |
32 |
– |
0.095 |
– |
– |
– |
– |
– |
– |
|
| 5 | 1st passage CWDWTD in vole (CWDVole-P1) |
13 | – | 4.000 | + | + | – | NA | – | – |
| 6 | 18 | – | 4.000 | + | + | +/− | – | – | – | |
| 7 | 22 | – | 4.000 | + | + | + | – | – | – | |
| 8 | 22 | + | 4.000 | + | + | + | + | – | – | |
| 9 |
24 |
+ |
4.000 |
+ |
+ |
+ |
+ |
– |
– |
|
| 10 | 5th passage CWDWTD in vole (CWDVole-P5) |
3 | – | 0.093 | – | – | – | – | – | NA |
| 11 | 17 | – | 4.000 | + | + | + | NA | – | – | |
| 12 | 18 | + | 4.000 | + | + | + | + | – | – | |
| 13 | 21 | + | 4.000 | + | + | + | + | – | – | |
| 14 |
21 |
+ |
4.000 |
+ |
+ |
+ |
+ |
– |
– |
|
| 15 | CWD-negative WTD in vole (CWDNeg) | 35 | – | 0.097 | – | – | – | – | – | – |
| 16 | 35 | – | 0.095 | – | – | – | – | – | – | |
| 17 | 35 | – | 0.090 | – | – | – | – | – | – | |
| 18 | 35 | – | 0.109 | – | – | – | – | – | – | |
*CWD, chronic wasting disease; EIA, antigen-capture enzyme immunoassay ; ENS, enteric nervous system; LRS, lymphoid tissues; mpi, months postinoculation; NA, not available; OD, optical density; WTD, white-tailed deer.