Table 2. HRTV-positive Amblyomma americanum tick pools from Putnam and Jones Counties, Georgia, 2019*.

No. pools	Collection date	Site	No. specimens in pool	HRTV real-time PCR result for homogenate	Vero E6 cells
					P1			P2			P3
					CPE	PCR		CPE	PCR		CPE	PCR
23	Apr 28	1	5	+	‒	‒		‒	‒		‒	‒
26	Apr 28	1	25	+	+	+		‒	+		‒	‒
504	Jun 14	2	5	+	‒	+		‒	+		‒	+

*CPE, cytopathic effect; HRTV, Heartland virus; P1, primary passage; P2, subculture 2; P3, subculture 3. ‒ negative; +, positive.