FIG. 1.

Schematic representation of the strategy used for the generation of CYP51(R467K). Site-directed PCR mutagenesis was performed to change the triplet at position 467 from AGA to GGA as described in Materials and Methods. The NsiI-HindIII mutant fragment was ligated into the corresponding region of the wild-type gene in the yeast expression vector YEp51 allowing expression from the GAL10 promoter.