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Reduced-carbon monoxide difference spectrum of microsomal cytochrome CYP51(R467K). Shown are the reduced-carbon monoxide difference spectra of microsomal fractions, prepared as described in Materials and Methods, after induction of heterologous expression in yeast transformants containing YEp51 alone (— • —), CYP51 (——), and CYP51 (R467K) (–––).
