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. 2022 Mar 11;12:837589. doi: 10.3389/fonc.2022.837589

Figure 5.

Figure 5

Temozolomide stimulates ciliogenesis in L0 and S7 cells. (A) S7 and (B) L0 cells were grown for 24 h in vehicle or 10 µM TMZ. Cells were fixed and immunostained for OFD1 (red) and ARL13B (green). Nuclei were labeled with DAPI. TMZ-treated cells appeared to show elongated ARL13B+ cilia (arrowheads) and OFD1 intensity at the base of the cilia (arrows) appeared to decrease compared with vehicle in both cell lines. Scale bar = 10 µm. Mean lengths of cilia in (C) S7 and (D) L0 cells after a 24-h exposure to the indicated concentration (µM) of TMZ. (E) S7 and (F) L0 cells were grown for 72 h after one treatment of vehicle, 10 or 50 µM TMZ. Cells were fixed and immunostained for OFD1 (red) and ARL13B (green). Arrows point to ARL13B+ cilia in each treatment. Mean lengths of ARL13B+ cilia in (G) S7 or (I) L0 cells 3 days after exposure to the indicated concentration (µM) of TMZ. Percent of ARL13B+ cilia in (H) S7 or (J) L0 cells 3 days after exposure to the indicated concentration (µM) of TMZ. *p < 0.05, **p < 0.01, ***p < 0.005, ****p < 0.0001 (ANOVA).