Figure 6.

TTFields blocks the TMZ-mediated increase in ciliogenesis. Adherent S7 cells pretreated with (A, C) vehicle or (B, D) 10 µM TMZ and left untreated (control) (A, B) or exposed to 3 days of TTFields (C, D). Adherent L0 cells pretreated with (E, G) vehicle or (F, H) 20 µM TMZ and left untreated (control) (E, F) or exposed to 3 days of TTFields (G, H). S7 and L0 cells were fixed and stained for ARL13B (green) and OFD1 (red). Nuclei are labeled with DAPI (blue). Spheres of S7 cells pretreated with (I, K) vehicle or (J, L) 50 µM TMZ and left untreated (control) (I, J) or exposed to 3 days of TTFields (K, L). To examine cilia, spheres were fixed, sectioned, and stained for ARL13B (green, arrows). The number of cilia/per area (µm²) of traced sections of (M) S7 or (O) L0 spheres for the indicated treatment. The mean length (µm) of cilia in (N) S7 or (P) L0 spheres for the indicated treatment. ^* p < 0.05; ^** p < 0.01; ^*** p < 0.001; ^**** p < 0.0001 (ANOVA). Scale bars (in µm) in (A) = 10 and (I) = 20.