Skip to main content
. Author manuscript; available in PMC: 2022 Mar 29.
Published in final edited form as: Cell Stem Cell. 2020 Nov 11;28(2):315–330.e5. doi: 10.1016/j.stem.2020.10.006

KEY RESOURCES TABLE.

REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
Rabbit monoclonal anti-Cytokeratin 10; 1:100 Abcam Cat# ab76318, RRID: AB_1523465
Rabbit polyclonal anti-Keratin 5; 1:1000 Biolegend Cat# 905501, RRID: AB_2565050
Rabbit monoclonal anti-Vimentin; 1:200 Abcam Cat# ab92547, RRID: AB_10562134
Rabbit monoclonal anti-α-Smooth Muscle Actin (Acta2); 1:100 Cell Signaling Technology Cat# 19245, RRID: 2734735
Rat monoclonal anti-CD31 (PECAM); 1:100 BD Biosciences Cat# 553370, RRID: AB_394816
Rabbit monoclonal anti-Myelin basic protein (MBP); 1:50 Cell Signaling Technology Cat# 78896
Goat polyclonal anti-Sox2; 1:100 Neuromics Cat# GT15098, RRID: AB_2195800
Chicken polyclonal anti-GFP; 1:500 Abcam Cat# ab13970, RRID: AB_300798
Goat anti-Chicken IgY (H+L), Alexa Fluor 488; 1:250 Thermo Fisher Scientific Cat# A-11039, RRID: AB_2534096
Goat anti-Rabbit IgG (H+L) Cross-Adsorbed, Alexa Fluor 555; 1:250 Thermo Fisher Scientific Cat# A-21428, RRID: AB_2535849
Goat anti-Rat IgG (H+L) Cross-Adsorbed, Alexa Fluor 488; 1:250 Thermo Fisher Scientific Cat# A-11006, RRID: AB_2534074
Donkey anti-Goat IgG (H+L) Cross-Adsorbed; 1:250 Thermo Fisher Scientific Cat# A-11055, RRID: AB_2534102
Biological Samples
Healthy tympanic membrane tissue for scRNA-Seq, from 34-year-old female University of California, San Francisco Under IRB approval
Undiseased tympanic membrane tissue used to derive fibroblasts for organoid experiments, from 55-year-old male University of California, San Francisco Under IRB approval
Undiseased tympanic membrane tissue used to derive keratinocytes for organoid experiments, from 27-year-old female University of California, San Francisco Under IRB approval
Chemicals
5-Ethynyl-2’-deoxyuridine (EdU) Carbosynth Limited NE08701
Crenolanib MedChem Express Cat# HY-13223; CAS No: 670220–88–9
CP-673451 Tocris Cat# 5993; CAS No: 343787–29–1
Palbociclib MedChem Express Cat# HY-50767; CAS No: 571190–30–2
Thiazovivin Selleckchem Cat# S1459; CAS No: 1226056–71–8
Compound library Selleckchem Details in Table S9
BGJ398 Cayman Chemical Cat# 19157; CAS No: 872511–34–7
PRN1371 MedChem Express Cat# HY-101768; CAS No: 1802929–43–6
HC-067047 MedChem Express Cat# HY-100208; CAS No: 883031–03–6
SB-525334 Cayman Chemical Cat# 16281; Cas No: 356559–20–1
GW788388 Selleckchem Cat# S2750; Cas No: 452342–67–5
Critical Commercial Assays
Dead Cell Removal Kit Miltenyi Biotec Cat# 130–090–101
RNAscope Multiplex Fluorescent Reagent Kit v2 Advanced Cell Diagnostics Cat# 323100
Click-iT EdU Alexa Fluor 488 Imaging Kit Thermo Fisher Scientific Cat# C10337
Deposited Data
Raw scRNA-Seq datasets (4) This paper GEO: GSE128892
Experimental Models: Organisms/Strains
Mouse: FVB/NJ From: The Jackson Laboratory Cat# 001800, RRID: IMSR_JAX:001800
Mouse: Ki67-CreERT2; Mki67tm2.1(cre/ERT2)Cle/J From: The Jackson Laboratory Cat# 029803, RRID: IMSR_JAX:029803
Mouse: Krt5-CreERT2; Tg(KRT5-cre/ERT2)1Blh From: Sarah Knox MGI: 4358332
Mouse: R26R-Confetti; Gt(ROSA)26Sortm1(CAG-Brainbow2.1)Cle/J From: Ophir Klein RRID: IMSR_JAX:013731
Mouse: mT/mG; Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J From: Ophir Klein RRID: IMSR_JAX:007676
Mouse: Krt19-CreERT; Krt19tm1(cre/ERT)Ggu/J From: Julie Sneddon MGI: 3797107, RRID: IMSR_JAX:026925
Mouse: Pdgfra-EGFP; Pdgfratm11(EGFP)Sor/J From: Tien Peng RRID: IMSR_JAX007669
Software and Algorithms
Cell Ranger 2.0.0 10x Genomics https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/latest/installation
Seurat https://satijalab.org/seurat/
CellFindR https://github.com/kevyu27/CellFindR
R https://www.r-project.org/
Fiji (ImageJ) https://fiji.sc/
Imaris Bitplane http://www.bitplane.com/