|
Antibodies
|
| Rabbit monoclonal anti-Cytokeratin 10; 1:100 |
Abcam |
Cat# ab76318, RRID: AB_1523465 |
| Rabbit polyclonal anti-Keratin 5; 1:1000 |
Biolegend |
Cat# 905501, RRID: AB_2565050 |
| Rabbit monoclonal anti-Vimentin; 1:200 |
Abcam |
Cat# ab92547, RRID: AB_10562134 |
| Rabbit monoclonal anti-α-Smooth Muscle Actin (Acta2); 1:100 |
Cell Signaling Technology |
Cat# 19245, RRID: 2734735 |
| Rat monoclonal anti-CD31 (PECAM); 1:100 |
BD Biosciences |
Cat# 553370, RRID: AB_394816 |
| Rabbit monoclonal anti-Myelin basic protein (MBP); 1:50 |
Cell Signaling Technology |
Cat# 78896 |
| Goat polyclonal anti-Sox2; 1:100 |
Neuromics |
Cat# GT15098, RRID: AB_2195800 |
| Chicken polyclonal anti-GFP; 1:500 |
Abcam |
Cat# ab13970, RRID: AB_300798 |
| Goat anti-Chicken IgY (H+L), Alexa Fluor 488; 1:250 |
Thermo Fisher Scientific |
Cat# A-11039, RRID: AB_2534096 |
| Goat anti-Rabbit IgG (H+L) Cross-Adsorbed, Alexa Fluor 555; 1:250 |
Thermo Fisher Scientific |
Cat# A-21428, RRID: AB_2535849 |
| Goat anti-Rat IgG (H+L) Cross-Adsorbed, Alexa Fluor 488; 1:250 |
Thermo Fisher Scientific |
Cat# A-11006, RRID: AB_2534074 |
| Donkey anti-Goat IgG (H+L) Cross-Adsorbed; 1:250 |
Thermo Fisher Scientific |
Cat# A-11055, RRID: AB_2534102 |
|
Biological Samples
|
|
|
| Healthy tympanic membrane tissue for scRNA-Seq, from 34-year-old female |
University of California, San Francisco |
Under IRB approval |
| Undiseased tympanic membrane tissue used to derive fibroblasts for organoid experiments, from 55-year-old male |
University of California, San Francisco |
Under IRB approval |
| Undiseased tympanic membrane tissue used to derive keratinocytes for organoid experiments, from 27-year-old female |
University of California, San Francisco |
Under IRB approval |
|
Chemicals
|
| 5-Ethynyl-2’-deoxyuridine (EdU) |
Carbosynth Limited |
NE08701 |
| Crenolanib |
MedChem Express |
Cat# HY-13223; CAS No: 670220–88–9 |
| CP-673451 |
Tocris |
Cat# 5993; CAS No: 343787–29–1 |
| Palbociclib |
MedChem Express |
Cat# HY-50767; CAS No: 571190–30–2 |
| Thiazovivin |
Selleckchem |
Cat# S1459; CAS No: 1226056–71–8 |
| Compound library |
Selleckchem |
Details in Table S9 |
| BGJ398 |
Cayman Chemical |
Cat# 19157; CAS No: 872511–34–7 |
| PRN1371 |
MedChem Express |
Cat# HY-101768; CAS No: 1802929–43–6 |
| HC-067047 |
MedChem Express |
Cat# HY-100208; CAS No: 883031–03–6 |
| SB-525334 |
Cayman Chemical |
Cat# 16281; Cas No: 356559–20–1 |
| GW788388 |
Selleckchem |
Cat# S2750; Cas No: 452342–67–5 |
|
Critical Commercial Assays
|
| Dead Cell Removal Kit |
Miltenyi Biotec |
Cat# 130–090–101 |
| RNAscope Multiplex Fluorescent Reagent Kit v2 |
Advanced Cell Diagnostics |
Cat# 323100 |
| Click-iT EdU Alexa Fluor 488 Imaging Kit |
Thermo Fisher Scientific |
Cat# C10337
|
|
Deposited Data
|
| Raw scRNA-Seq datasets (4) |
This paper |
GEO: GSE128892
|
|
Experimental Models: Organisms/Strains
|
| Mouse: FVB/NJ |
From: The Jackson Laboratory |
Cat# 001800, RRID: IMSR_JAX:001800 |
| Mouse: Ki67-CreERT2; Mki67tm2.1(cre/ERT2)Cle/J |
From: The Jackson Laboratory |
Cat# 029803, RRID: IMSR_JAX:029803 |
| Mouse: Krt5-CreERT2; Tg(KRT5-cre/ERT2)1Blh |
From: Sarah Knox |
MGI: 4358332 |
| Mouse: R26R-Confetti; Gt(ROSA)26Sortm1(CAG-Brainbow2.1)Cle/J |
From: Ophir Klein |
RRID: IMSR_JAX:013731 |
| Mouse: mT/mG; Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J |
From: Ophir Klein |
RRID: IMSR_JAX:007676 |
| Mouse: Krt19-CreERT; Krt19tm1(cre/ERT)Ggu/J |
From: Julie Sneddon |
MGI: 3797107, RRID: IMSR_JAX:026925 |
| Mouse: Pdgfra-EGFP; Pdgfratm11(EGFP)Sor/J |
From: Tien Peng |
RRID: IMSR_JAX007669 |
|
Software and Algorithms
|
| Cell Ranger 2.0.0 |
10x Genomics |
https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/latest/installation
|
| Seurat |
|
https://satijalab.org/seurat/
|
| CellFindR |
|
https://github.com/kevyu27/CellFindR
|
| R |
|
https://www.r-project.org/
|
| Fiji (ImageJ) |
|
https://fiji.sc/
|
| Imaris |
Bitplane |
http://www.bitplane.com/
|
