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. 2022 Feb 21;14(4):839. doi: 10.3390/polym14040839

Figure 10.

Figure 10

The strategy of 3D bioprinting with double cross-linking steps was as follows: the first cross-linking step involved the partial cross-linking of HA–ADH–biotin–streptavidin (HBS) hydrogel, and the HBS mixed with sodium alginate served as a hybrid bioink (HBSA) for the second ionic cross-linking step with Ca2+ ions. The 3D HBSA scaffolds after printing were submerged in CaCl2 solution to achieve ionic cross-linking to form an HBSAC hydrogel construct through ion transfer (reprinted with the permission from [99]).