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. Author manuscript; available in PMC: 2023 Apr 1.
Published in final edited form as: Gastroenterology. 2022 Jan 6;162(4):1319–1333. doi: 10.1053/j.gastro.2021.12.281

Figure 2. The PSCA CAR_s15 vector enhances IFN-γ secretion and tumor lytic functions of NK cells in vitro.

Figure 2.

(A) The median of fluorescence intensity (MFI) and the percentage of PSCA(+) cells in the indicated PC cell lines. (B) IFN-γ levels in the supernatants of co-cultured engineered NK cells and pancreatic tumor cells, as measured by enzyme-linked immunosorbent assay (ELISA). Statistical analysis was performed by one-way ANOVA. (C, D) Cytotoxicity of engineered NK cells against (C) PSCA(+) Capan-1 or (D) PSCA(–) PANC-1 tumor cells, at an effector: target (E:T) ratio of 1:3 measured after 48 hours of co-culture. (E, F) Real-time cell analysis (RTCA) of the cytotoxicity of engineered NK cells against (E) PSCA(+) Capan-1 or (F) PSCA(–) PANC-1 tumor cells with an E:T ratio of 1:3 and presented as the growth index of the residual cancer cells. The tumor only control group contained no NK cells. s15 = s15 NK cells; PSCA = PSCA CAR NK cells; PSCA-s15 = PSCA CAR_s15 NK cells.