Skip to main content
. 2022 Mar 11;13:817951. doi: 10.3389/fphar.2022.817951

FIGURE 7.

FIGURE 7

Intracardiac ABCB8 mRNA injection prevents mitochondrial DOX retention and cardiotoxicity despite cardiac iron overload. Hfe+/+ and Hfe−/− mice received ABCB8 mRNA (15 µg) and DOX (20 mg/kg) as described in the Methods section. ABCB8 expression was determined by western blot analysis and normalized to α-tubulin levels (A,B). Cardiac non-heme iron levels were measured by colorimetric assay using bathophenanthroline disulfonate (C). Mitochondrial (D) and cardiac (E) DOX levels were measured in cardiac tissue by fluorescence and normalized to protein content. Oxidative stress was determined as TBARS levels and measured by colorimetric assay (F). Serum CK-MB levels were measured by ELISA (G). Results are representative of n = 6–7/group except (A) where n = 4-5/group. Data were expressed as mean ± SEM. Statistical significance was analyzed by ANOVA followed by Tukey’s post-hoc comparisons. *p < .05 vs. Hfe+/+ of the same treatment. # p < .05 vs. mRNA control of the same genotype.