FIGURE 2.

Aldosterone treatment increases SR Ca²⁺ content and does not change global [Ca²⁺]_cyt in Fura 2-loaded MASMCs. (A) Scatterplot with mean ± SEM illustrates basal [Ca²⁺]_cyt before caffeine challenge determined in Fura 2-loaded MASMCs, treated (red circles, n = 16 cells/N = 5 rats) or not (empty circles, n = 16 cells/N = 5 rats) with aldosterone (ALDO 10 nM, 24 h). (B) Representative recordings of caffeine-induced [Ca²⁺] transients in Fura 2-loaded MASMCs treated (ALDO, red trace) or not (CONTROL, black trace) with aldosterone (10 nM, 24 h). (C) Scatterplot with mean ± SEM of caffeine-induced Ca²⁺ transient amplitude recorded at the peak of the Ca²⁺ transient in MASMCs treated (red circles, n = 16 cells/N = 5 rats) or not (empty circles, n = 16 cells/N = 5 rats) with ALDO. ^***P < 0.001 vs. control MASMCs.