FIGURE 6.

Enhanced acetylcholine-mediated vasorelaxation of ALDO-treated MAs. Relaxation responses of MAs treated (ALDO, red circles) or not (CONTROL, empty circles) with aldosterone (10 nM, 24 h) to increasing concentrations of acetylcholine (ACh, from 0.001 to 1 μM). MA rings were pre-contracted with KCl 60 mM and exposed to cumulative ACh concentrations (n = 6 MA rings/N = 6 rats, for each experimental condition). Relaxation is shown as a percentage with respect to the contraction response produced by 60 mM KCl, which was taken as 100%. Data are shown as mean ± SEM. *P < 0.05 vs. non-linear data fit of control arteries.