TABLE 1.

List of primer sequences for real-time qPCR.

Target gene	Sequence accession number	Primer	Tm (°C)	Sequence	Product length (pb)
Atp2a2	NM_001110139.2	F R	59.0 59.1	5′- ACCTGGAAGATTCTGCGAAC -3′ 5′- AATCCTGGGAGGGTCCAG -3′	86
Sgk1	NM_019232.3	F R	60.3 58.4	5′- CTGCTCGAAGTACCCTCACC -3′ 5′- GCATGCATAGGAGTTGTTGG -3′	128
Orai1 Stim1	NM_001013982.1 NM_001108496.2	F R F R	59.49 59.88 59.54 59.63	5′- ATCGTCTTTGCCGTTCACTT -3′ 5′- AGAGAATGGTCCCCTCTGTG -3′ 5′- TCTCTGAGTTGGAGGATGAGTAGA -3′ 5′- CAATATAGGGGAGCAGAGGTAAGA -3′	131 112
Tagln	NM_031549.2	F R	60.5 62.2	5′- GTTTGGCCGTGACCAAGAAC -3′ 5′- GGAGGCCAATGACGTGCTTC -3′	129
Rpl32	NM_013226.2	F R	58.6 58.9	5′- GCTGCTGATGTGCAACAAA -3′ 5′- GGGATTGGTGACTCTGATGG -3′	115
Ywhaz	NM_013011.3	F R	60.3 54.7	5′- AGACGGAAGGTGCTGAGAAA -3′ 5′- GAAGCATTGGGGATCAAGA -3′	127

Atp2a2, ATPase sarcoplasmic/endoplasmic reticulum Ca²⁺ transporting 2 or SERCA pump; Sgk1, serum/glucocorticoid-regulated kinase; Orai1, calcium release-activated calcium channel protein 1; Stim1, stromal interaction molecule 1; Tagln, transgelin also known as smooth muscle cell 22 alpha; Rpl32, ribosomal protein L32; Ywaz, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta; F, forward; R, reverse; Tm, melting temperature; pb, pair bases. Information obtained from MFEprimer 3.1 (Wang et al., 2019).