FIGURE 5.

H. lanceolatum aqueous extract possesses an anti-estrogenic activity. (A) Quantification of GFP fluorescence and (B) representative pictures of embryos exposed to water (Control) and different concentrations of H. lanceolatum aqueous extract (g/L) for 96 h (n = 26–38/group from 2 independent experiments). * denotes significant differences as compared to the control group (Dunn’s multiple comparison test, *p = 0.0438 and ****p < 0.0001). (C) Fluorescence quantification of GFP in embryos treated with control solvent (DMSO) and E2 at two different concentrations (2 and 10 nM) alone or co-exposed with H. lanceolatum aqueous extract 0.156 g/L (n = 19–21/group). * denotes significant differences between groups (Dunnett’s multiple comparison test, **p = 0.0011 and ***p = 0.0002). All the results are expressed as fold induction above control (means ± SEM).