Figure 3.

Phylogenetic relationship of 161 Persea americana (West-Indian pure accession) Nucleotide binding domains from putative PaNLR genes. Evolutionary history was inferred using the Maximum likelihood method and JTT matrix-based model following ClustalW alignment. A total of 1,000 bootstrap replicates were performed, with bootstrap values over 50 being shown above branch points. NB-domain protein sequences of Persea americana (PC) with complete NLR sequences from Cinnamomum micranthum f. kanehirae (RWR) and Solanum bulbocastanum (Q) were used during the analysis. Persea americana identification numbers include the gene cluster number, where appropriate (in blue) and protein type (in red). Unidentified PaNLR protein types were termed Disease resistance-like (DRL) proteins. Sequences from other species also include protein type (NBS50, NBS-LRR disease resistance protein NBS50; PDR, disease resistance-like protein isoform X1).