TABLE 2.

Estimation of geneflow in a triplet species following four-taxon tests with HybridCheck version 1.0 software.

Species in triplet			Outgroup species	Block start	Block end	numABBA	numBABA	D-statistic	Z-score	Direction of geneflow
P1	P2	P3	A
WR24	WR81	WR44	Nipponbare	1	1,118	168	10	0.47	0.35*	P3→P1
WR24	WR81	WR52	Nipponbare	1	1,118	133	6	0.38	−0.38*	P3→P1
WR24	WR81	WR62	Nipponbare	1	1,118	66	18	0.06	−1.81*	P3→P1
WR24	WR81	WR153	Nipponbare	1	1,118	73	18	0.03	−2.14*	P3→P1
WR24	WR81	WR161	Nipponbare	1	1,118	55	0	0.33	−0.78*	P3→P1
WR24	WR81	WR44	Nipponbare	1,119	2,236	0	8	–0.28	0.35*	P2→P3
WR24	WR81	WR52	Nipponbare	1,119	2,236	0	13	–0.49	−0.38*	P2→P3
WR24	WR81	WR62	Nipponbare	1,119	2,236	0	27	–0.69	−1.81*	P2→P3
WR24	WR81	WR153	Nipponbare	1,119	2,236	0	15	–0.49	−2.14*	P2→P3
WR24	WR81	WR161	Nipponbare	1,119	2,236	0	14	–0.43	−0.78*	P2→P3
WR24	WR81	WR44	Nipponbare	2,237	3,354	9	19	–0.57	0.35*	P2→P3
WR24	WR81	WR52	Nipponbare	2,237	3,354	9	16	–0.65	−0.38*	P2→P3
WR24	WR81	WR62	Nipponbare	2,237	3,354	9	19	–0.66	−1.81*	P2→P3
WR24	WR81	WR153	Nipponbare	2,237	3,354	9	19	–0.68	−2.14*	P2→P3
WR24	WR81	WR44	Nipponbare	3,355	4,472	19	21	–0.06	0.35*	P2→P3
WR24	WR81	WR52	Nipponbare	3,355	4,472	19	21	–0.12	−0.38*	P2→P3
WR24	WR81	WR62	Nipponbare	3,355	4,472	19	21	–0.16	−1.81*	P2→P3
WR24	WR81	WR153	Nipponbare	3,355	4,472	19	21	–0.31	−2.14*	P2→P3
WR24	WR81	WR161	Nipponbare	3,355	4,472	19	21	–0.14	−0.78*	P2→P3

Triplets are characterised: P1 = O. rufipogon type taxa; P2 = O. meridionalis; and P3 = Hybrid. O. sativa spp. japonica cv. Nipponbare (A) is an outgroup species. The length of aligned nucleotide is 4,473 bp containing four blocks: (1–1,118); (1,119–2,236); (2,237–3,354); and (3,355–4,473). The biallelic patterns based on the number of ABBA-BABA in a triplet used in the Patterson’s D-statistic are shown. P3→P1 denotes geneflow between O. rufipogon type taxa and hybrid while P2→P3 denotes geneflow between O. meridionalis and hybrid. *Z-score represents statistically significant at p < 0.001.