Figure 3.

Motor tests of the behavior workflow allow for differentiation of tMCAO from sham and Control animals in different locomotor tasks. (A) Table comparing the parameters evaluated in the motor tests to the scores assessed in both the mRS and the NIHSS. (B) Schematic representation of the pole test. Time, in seconds, the mice took to both turn and descend a vertical pole presented in the graph [CT n = 4 (4 females), sham n = 14 (5 males and 9 females), tMCAO n = 14 (9 males and 5 females); statistical meaning over time to descend]. (C) Schematic representation of the rotarod test. The time the mice spent on the rotarod post-surgery was normalized to baseline values and presented in the graph [CT n = 4 (4 females), sham n = 14 (5 males and 9 females), tMCAO n = 14 (9 males and 5 females)]. (D) Schematic representation of the cylinder test. Cylinder's asymmetry score (difference between ipsilateral and contralateral overall limb use) allows to determine of which paw the mice preferentially use while weight-bearing [CT n = 12 (8 males and 4 females), sham n = 17 (5 males and 12 females), tMCAO n = 17 (9 males and 8 females)]. (E) Schematic representation of the ladder rung crossing and digit score evaluation. The ladder rung allows for evaluation of mice's misplacement of the paws (error frequency as a percent of total steps) and fine motor abilities (digit scores from 0 to 2) [CT n = 4 (4 females), sham n = 14 (5 males and 9 females), tMCAO n = 14 (9 males and 5 females)]. (F) Schematic representation of the open field arena. The open field paradigm allows for discrimination of mice locomotor gait [distance, resting time, speed, and turn propensity (left/right turn ratio)] [CT n = 12 (8 males and 4 females), sham n = 14 (5 males and 9 females), tMCAO n = 14 (9 males and 5 females)]. ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.05. One-way ANOVA or two-way ANOVA followed by Tukey's multiple comparison t-test.