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. 2022 Mar 29;17(3):e0266135. doi: 10.1371/journal.pone.0266135

Fig 4. Gingerenone A suppresses SASP and enhances apoptosis in senescent cells.

Fig 4

(A) WI-38 fibroblasts were rendered senescent by exposure to ionizing radiation (IR, 10 Gy) and cultured for an additional 10 days. Cells were then treated with gingerenone A (20 μM) and 6-shogaol (72.4 nM) and incubated for 24 h. CCL-2 (MCP-1) (green) and IL-10 (grey) were measured using the Bio-plex (Multiplex); IL6 (pink) was measured using Quantikine IL-6 (R&D). Data were normalized to cell number/total protein content. Data in graphs represent the means and standard error from three biological replicates. (B) Western blot analysis of the proteins shown after treating proliferating and senescent fibroblasts (as explained in panel A) for 48 h with the drugs indicated. Ratios of cleaved pro-caspase 3 to total caspase 3, normalized to ACTB levels, shown as relative to control proliferating cells.