Figure 1.

Photographs of the bioreactor used to perfuse decellularized scaffolds with whole blood. (A) Image outlining the arterial line (which was then attached to the cannulated renal artery) and venous lines (which was left open to act as a venous reservoir to facilitate fluid recirculation) before the addition of the scaffold. (B) Image of an acellular kidney perfused with PBS illustrates how the scaffold recirculated fluid that emanated from its renal vein (red arrow) and open-ended venous line. (C) Image of a scaffold being perfused with whole pig blood.