| Strain, strain background (Mus musculus) |
miR-138-flox |
Taconic Artemis GmbH (Cologne, Germany) |
C57BL/6NTac-Gt(ROSA)26So tm2459(LacZ, antimir_138) Arte
|
|
| Strain, strain background (M. musculus) |
CMV-Cre |
Jackson Laboratories |
B6.C-Tg(CMV-Cre)1Cgn (CMV-CRE) |
|
| Strain, strain background (M. musculus) |
PV-Cre |
Jackson Laboratories |
B6;129P2-Pvalbtm1(cre)Arbr/J (PV-CRE) |
|
| Recombinant DNA reagent |
pAAV-6P-SEWB (modified) |
Christensen et al., 2010
|
|
Used for cloning of miR-138 sponge plasmids |
| Recombinant DNA reagent |
pAAV-mDlx-GFP-Fishell-1 |
Addgene, 83900 |
|
Used for cloning of mDlx-miR-138 sponge plasmids |
| Recombinant DNA reagent |
pGL3-promoter vector |
Promega, Mannheim |
|
Used for cloning of miR-138 perfect binding site reporter |
| Recombinant DNA reagent |
C1-mCherry |
Addgene, 632524 |
|
Used for cloning of miRNA sensor plasmids |
| Recombinant DNA reagent |
AAV-hSyn-EGFP |
Addgene, 114213 |
|
Used for cloning of miRNA sensor plasmids |
| Recombinant DNA reagent |
pmirGLO dual-luciferase expression vector reporter |
Promega, Madison, WI |
|
Used for cloning of luciferase reporter constructs |
| Sequence-based reagent |
Mir-138 FISH probe (Fast Red) |
Thermo Fisher Scientific |
VM1-10093-VCP |
Sequence not provided by commercial supplier |
| Sequence-based reagent |
ErbB4 FISH probe (488) |
Thermo Fisher Scientific |
VC4-3146482-VC |
Sequence not provided by commercial supplier |
| Sequence-based reagent |
Rims3 FISH probe (488) |
Thermo Fisher Scientific |
VC4-3146880-VCP |
Sequence not provided by commercial supplier |
| Sequence-based reagent |
Camk2a FISH probe (488) |
Thermo Fisher Scientific |
VC6-11639-VCP |
Sequence not provided by commercial supplier |
| Sequence-based reagent |
Gad2 FISH probe (647) |
Thermo Fisher Scientific |
VC6-16451-VCP |
Sequence not provided by commercial supplier |
| Antibody |
aGAD65+67 (rabbit polyclonal) |
Abcam |
Ab11070 |
1:100 (in vitro); 1:1000 (ex vivo) |
| Antibody |
aMAP2 (Mouse monoclonal) |
Sigma-Aldrich |
M9942 |
1:1000 |
| Antibody |
aGFAP (rabbit polyclonal) |
Dako |
Z0334 |
1:1000 |
| Antibody |
aParvalbumin (mouse monoclonal) |
SWANT |
235 |
1:1000 |
| Antibody |
aVGAT (rabbit polyclonal) |
Synaptic systems |
131003 |
1:1000 |
| Antibody |
amCherry (rabbit polyclonal) |
Abcam |
Ab167453 |
1:1000 |
| Antibody |
aBeta galatosidase (chicken polyclonal) |
Abcam |
Ab9361 |
1:4000 |
| Antibody |
aMouse Alexa Fluor 488 (Donkey polyclonal) |
Invitrogen |
A21202 |
1:500 |
| Antibody |
aRabbit Alexa Fluor 546 (Goat polyclonal) |
Invitrogen |
A11010 |
1:500 |
| Antibody |
aChicken Alexa Fluor 546 (Goat polyclonal) |
Invitrogen |
A11040 |
1:500 |
| Antibody |
aMouse Alexa Fluor 647 (Donkey polyclonal) |
Invitrogen |
A31571 |
1:500 |
| Chemical compound, drug |
Hoechst 33342 |
Thermo Fisher Scientific |
62249 |
|
