| Cell line (Cricetulus griseus) |
ExpiCHO-S |
Gibco, Thermo Fisher Scientific (Waltham, MA) |
Cat. No. A29127 |
|
| Cell line (Homo sapiens) |
Expi293F |
Gibco, Thermo Fisher Scientific (Waltham, MA) |
Cat. No. A14527 |
|
| Antibody |
MAb362-IgG1(Human monoclonal) |
PMID:32826914
|
|
ELISA (600 nM),FCS (600 nM), smFRET (see Materials and methods section ‘smFRET imaging and data analysis’). |
| Antibody |
MAb362-IgA1(Human monoclonal) |
PMID:32826914
|
|
ELISA (600 nM),FCS (600 nM), smFRET (see Materials and methods section ‘smFRET imaging and data analysis’). |
| Antibody |
REGN10987(Mouse monoclonal) |
This work and PMID:32540901
|
|
See Materials and methods section ‘Antibodies’.ELISA (600 nM),FCS (600 nM), smFRET (see Materials and methods section ‘smFRET imaging and data analysis’). |
| Antibody |
S309(Human monoclonal) |
This work andPMID:32422645
|
|
See Materials and methods section ‘Antibodies’.ELISA (600 nM),FCS (600 nM), smFRET (see Materials and methods section ‘smFRET imaging and data analysis’). |
| Antibody |
CR3022(Human monoclonal) |
This work and PMID:16796401
|
|
See Materials and methods section ‘Antibodies’.ELISA (600 nM),FCS (600 nM), smFRET (see Materials and methods section ‘smFRET imaging and data analysis’). |
| Antibody |
2G12(Human monoclonal) |
This work |
|
See Materials and methods section ‘Antibodies’.ELISA (600 nM),FCS (600 nM), smFRET (see Materials and methods section ‘smFRET imaging and data analysis’). |
| Antibody |
4A8(Human monoclonal) |
BioVision (Milpitas, CA) |
Cat. No. A2269-100 |
ELISA (600 nM),FCS (600 nM), smFRET (see Materials and methods section ‘smFRET imaging and data analysis’). |
| Antibody |
1A9(Mouse monoclonal) |
GeneTex (Irvine, CA) |
Cat. No. GTX632604 |
ELISA (600 nM),FCS (600 nM),SmFRET: see Materials and methods section ‘smFRET imaging and data analysis’,WB (1:2000). |
| Antibody |
Anti-6x-His-tag(Rabbit polyclonal) |
Invitrogen (Waltham, MA) |
Cat. No. PA1-983B |
WB (1:2000). |
| Antibody |
HRP-conjugated anti-mouse IgG Fc(Rabbit polyclonal) |
Invitrogen (Waltham, MA) |
Cat. No. 31455 |
ELISA (1:5000), WB (1:5000). |
| Antibody |
HRP-conjugated anti-human IgG Fc(Goat polyclonal) |
Invitrogen (Waltham, MA) |
Cat. No. A18823 |
ELISA (1:10,000), WB (1:10,000). |
| Antibody |
HRP-conjugated anti-human kappa(Goat polyclonal) |
SouthernBiotech (Birmingham, AL) |
Cat. No. 2060–05 |
ELISA (1:4000). |
| Antibody |
HRP-conjugated anti-rabbit IgG(Goat polyclonal) |
Abcam (Cambridge, UK) |
ab205718 |
WB (1:50,000). |
| Recombinant DNA reagent |
pcDNA3.1_ REGN10987-heavy_chain |
This work and PMID:32540901
|
|
See Materials and methods section ‘Antibodies’. |
| Recombinant DNA reagent |
pcDNA3.1_ REGN10987-light_chain |
This work and PMID:32540901
|
|
See Materials and methods section ‘Antibodies’. |
| Recombinant DNA reagent |
pcDNA3.1_S309-heavy_chain |
This work andPMID:32422645
|
|
See Materials and methods section ‘Antibodies’. |
| Recombinant DNA reagent |
pcDNA3.1_S309-light_chain |
This work andPMID:32422645
|
|
See Materials and methods section ‘Antibodies’. |
| Recombinant DNA reagent |
pcDNA3.1_CR3022-heavy_chain |
This work and PMID:16796401
|
|
See Materials and methods section ‘Antibodies’. |
| Recombinant DNA reagent |
pcDNA3.1_CR3022-light_chain |
This work and PMID:16796401
|
|
See Materials and methods section ‘Antibodies’. |
| Recombinant DNA reagent |
Plasmid_2G12-heavy_chain |
Peter D Kwong laboratory |
|
|
| Recombinant DNA reagent |
Plasmid_2G12-light_chain |
Peter D Kwong laboratory |
|
|
| Recombinant DNA reagent |
pcDNA3.1 SARS-CoV-2 SΔTM (plasmid) |
This work.GenScript (Piscataway, NJ) |
|
See Materials and methods section ‘Plasmids and site-directed mutagenesis’. |
| Recombinant DNA reagent |
pcDNA3.1 SARS-CoV-2 SΔTM 161/345A4 double-tagged (plasmid) |
This work |
|
See Materials and methods section ‘Plasmids and site-directed mutagenesis’. |
| Recombinant DNA reagent |
pcDNA3.1 SARS-CoV-2 SΔTM D614G (plasmid) |
This work |
|
See Materials and methods section ‘Plasmids and site-directed mutagenesis’. |
| Recombinant DNA reagent |
pcDNA3.1 SARS-CoV-2 SΔTM D614G 161/345A4 double-tagged (plasmid) |
This work |
|
See Materials and methods section ‘Plasmids and site-directed mutagenesis’. |
| Recombinant DNA reagent |
pCAGGS-ACE2-his (plasmid) |
PMID:32991842
|
Addgene No. 158089 (Watertown, MA) |
|
| Peptide, recombinant protein |
SARS-CoV-2 SΔTM |
This work |
|
See Materials and methods section ‘Protein expression and purification’. |
| Peptide, recombinant protein |
SARS-CoV-2 SΔTM 161/345A4 double-tagged |
This work |
|
See Materials and methods section ‘Protein expression and purification’. |
| Peptide, recombinant protein |
SARS-CoV-2 SΔTM D614G |
This work |
|
See Materials and methods section ‘Protein expression and purification’. |
| Peptide, recombinant protein |
SARS-CoV-2 SΔTM D614G 161/345A4 double-tagged |
This work |
|
See Materials and methods section ‘Protein expression and purification’. |
| Peptide, recombinant protein |
ACE2 |
This work |
|
See Materials and methods section ‘Protein expression and purification’. |
| Peptide, recombinant protein |
Acyl carrier protein synthase (AcpS) |
PMID:31952255
|
|
|
| Commercial assay, kit |
Q5 Site-Directed Mutagenesis Kit |
New England Biolabs(Ipswich, MA) |
Cat. No. E0554S |
|
| Commercial assay, kit |
Coomassie Plus (Bradford) Assay Kit |
Thermo Fisher Scientific (Waltham, MA) |
Cat. No. 23,236 |
|
| Commercial assay, kit |
SuperSignal West Pico PLUS Chemiluminescent Substrate |
Thermo Scientific (Waltham, MA) |
Cat. No. 34580 |
|
| Commercial assay, kit |
1-Step Ultra TBM-ELISA |
Thermo Scientific (Waltham, MA) |
Cat. No. 34028 |
|
| Chemical compound, drug |
Cy5-conjugated n-hydroxysuccinimide ester |
Cytiva (Marlborough, MA) |
Cat. No. PA15100 |
|
| Chemical compound, drug |
Coenzyme A (CoA)-conjugated LD550 fluorophore |
Lumidyne Technologies (New York, NY), |
Cat. No. LD550-CS |
|
| Chemical compound, drug |
Coenzyme A (CoA)-conjugated LD650 fluorophore |
Lumidyne Technologies (New York, NY), |
Cat. No. LD650-CS |
|
| Software, algorithm |
Micromanager |
PMID:25606571micro-manager.org
|
v2.0 |
|
| Software, algorithm |
SPARTAN |
https://www.scottcblanchardlab.com/software and PMID:26878382
|
Version 3.7 |
|
| Software, algorithm |
Matlab (Mathworks, Natick, MA) |
Mathworks (Natick, MA) |
Version R2018b |
|
| Software, algorithm |
Maximum point likelihood algorithm |
PMID:11023897
|
|
|
| Software, algorithm |
GraphPad Prism |
GraphPad Software(San Diego, CA) |
Version 9.2.0 |
|
| Software, algorithm |
PyMOL software |
The PyMOL Molecular Graphic System, Schrödinger Inc(New York, NY) |
Version 2.0.7 |
|
| Other |
Pierce Protein G Agarose |
Thermo Fisher Scientific (Waltham, MA) |
Cat. No. 20398 |
|
| Other |
Ni-NTA Agarose |
Invitrogen (Waltham, MA) |
Cat. No. R901-15 |
|
| Other |
Superdex 200 Increase 10/300 GL column |
GE Healthcare (Chicago, IL) |
Cat. No. 28990944 |
|
| Other |
Gel Filtration Standard |
Bio-Rad (Hercules, CA) |
Cat. No. 1511901 |
|
| Other |
Synergy H1 microplate reader |
BioTek (Winooski, VT) |
|
|
| Other |
Typhoon 9,410variable mode imager |
GE Amersham Biosciences (Amersham, UK) |
|
|
| Other |
CorTector SX100 instrument |
LightEdge Technologies(Beijing, China) |
|
|
| Other |
QuantaMaster 400 fluorimeter |
Horiba (Kyoto, Japan) |
|
|
