Table 2. Förster resonance energy transfer (FRET)-state occupancies and rate constants for SΔTM D614.
Data are presented at mean ± standard error determined from the total population of traces analyzed.
| SARS-CoV-2 spikes D614 | FRET-state occupancies (%) | Rate constants (s–1) | ||
|---|---|---|---|---|
| Low-FRET (0.35)RBD-up conformation | High-FRET (0.65)RBD-down conformation | kdown(Low to high FRET) | kup(High to low FRET) | |
| Unbound | 40 ± 3 | 60 ± 3 | 3.8 ± 0.2 | 2.6 ± 0.2 |
| + ACE2 | 66 ± 3 | 34 ± 3 | 1.3 ± 0.1 | 2.2 ± 0.2 |
| + MAb362IgG1 | 52 ± 2 | 48 ± 2 | 2.2 ± 0.1 | 2.2 ± 0.1 |
| + MAb362IgA1 | 54 ± 2 | 46 ± 2 | 1.8 ± 0.1 | 2.0 ± 0.1 |
| + REGN10987 | 52 ± 3 | 48 ± 3 | 2.4 ± 0.2 | 2.2 ± 0.2 |
| + S309 | 49 ± 3 | 51 ± 3 | 2.3 ± 0.1 | 2.1 ± 0.1 |
| + CR3022 | 54 ± 4 | 46 ± 4 | 2.3 ± 0.2 | 3.1 ± 0.2 |
| + 4A8 | 48 ± 2 | 52 ± 2 | 2.3 ± 0.1 | 2.3 ± 0.1 |
| + 1A9 | 60 ± 2 | 40 ± 2 | 2.2 ± 0.1 | 3.4 ± 0.2 |
| + 2G12 | 59 ± 2 | 41 ± 2 | 2.5 ± 0.1 | 3.4 ± 0.2 |