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. 2022 Mar 18;11:e74782. doi: 10.7554/eLife.74782

Figure 4. Low Pi feeding limits functional iron deficiency in Col4a3−/− (Alport ) mice.

BUN, serum creatinine (A), serum FGF23 and serum Pi levels (B). Quantitative polymerase chain reaction (qPCR) analysis of Il1b, Il6, and Saa1 (C, D) and Hamp (E) expression levels in liver tissue. (F) CBC analysis. (G) Representative gross pathology of Perls’ Prussian blue-stained spleen sections (scale bar, 50 μm). Larger magnification is shown in supplementary figure and legends. (H) Liver Pi levels. All values are mean ± standard error of the mean (SEM; n = 7–9 mice/group; *p ≤ 0.05 vs. Col4a3+/+ + 0.6% Pi diet, #p ≤ 0.05 vs. Col4a3−/− + 0.6% Pi diet) where statistical analyses were calculated by two-way analysis of variance (ANOVA) followed by Tukey’s multiple comparison post hoc test. Dotted lines indicate median Col4a3+/+ + 0.6% Pi diet measurements.

Figure 4.

Figure 4—figure supplement 1. Low Pi feeding limits functional iron deficiency in Col4a3−/− (Alport ) mice.

Figure 4—figure supplement 1.

(A) Representative gross pathology of H&E-stained kidney sections (original magnification, ×20; scale bar, 50 μm) from wild-type (Col4a3+/+) and Alport (Col4a3−/−) mice, fed either control diet (0.6% Pi) or a low Pi diet (0.2% Pi). Pathologic changes were detected in sections stained with H&E from Alport mice fed a 0.6% Pi diet. A 0.2% Pi diet moderately improves this feature in Alport mice. (B) Hematocrit percentage (HCT%), mean corpuscular hematocrit (MCH), and serum transferrin saturation percentage (TSAT%) analysis in wild-type (Col4a3+/+) and Alport (Col4a3−/−) mice, fed either control diet (0.6% Pi) or a low Pi diet (0.2% Pi). (C) Spleen nonheme iron concentrations in wild-type (Col4a3+/+) and Alport (Col4a3−/−) mice, fed either control diet (0.6% Pi) or a low Pi diet (0.2% Pi). (D) Liver nonheme iron concentrations in wild-type (Col4a3+/+) and Alport (Col4a3−/−) mice, fed either control diet (0.6% Pi) or a low Pi diet (0.2% Pi). (E) Representative gross pathology of Perls’ Prussian blue-stained spleen sections (original magnification, ×40; scale bar, 20 μm). All values are mean ± standard error of the mean (SEM; n = 7–9 mice/group; *p ≤ 0.05 vs. Col4a3+/+ + 0.6% Pi diet, #p ≤ 0.05 vs. Col4a3−/− + 0.6% Pi diet) where statistical analyses were calculated by two-way analysis of variance (ANOVA) followed by Tukey’s multiple comparison post hoc test. Dotted lines indicate corresponding median measurements from Col4a3+/+ mice on 0.6% Pi diet.