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. 2022 Mar 18;11:e74782. doi: 10.7554/eLife.74782

Figure 7. Pi induces hepcidin expression via paracrine IL1β and IL6 signaling.

Quantitative polymerase chain reaction (qPCR) analysis of Il1b, Il6 (A) and Hamp (B) expression levels in primary hepatocytes following stimuli, with or without BAY 11-7082; values are mean ± standard error of the mean (SEM; n = 4 independent isolations; *p ≤ 0.05 vs. vehicle control [Ctrl], #p ≤ 0.05 vs. 20 μM BAY 11-7082 Ctrl). Dotted lines indicate median vehicle Ctrl measurements. (C) qPCR analysis of Hamp expression levels in primary hepatocytes following stimuli with or without anti-IL1β, anti-IL6, or both antibodies in combination; values are mean ± standard error of the mean (SEM; n = 4 independent isolations; *p ≤ 0.05 vs. vehicle control [Ctrl], #p ≤ 0.05 vs. anti-IL1β Ctrl, $p ≤ 0.05 vs. anti-IL6 Ctrl, @p ≤ 0.05 vs. anti-IL1β + anti-IL6 Ctrl) where statistical analyses were calculated by two-way analysis of variance (ANOVA) followed by Tukey’s multiple comparison post hoc test. Dotted lines indicate median vehicle Ctrl measurements.

Figure 7.

Figure 7—figure supplement 1. Pi induces hepcidin expression via paracrine IL1β and IL6 signaling.

Figure 7—figure supplement 1.

Quantitative polymerase chain reaction (qPCR) analysis of primary hepatocytes shows expression levels of Saa1 (A), Hp (B), and Slc20a1 (C) following lipopolysaccharide (LPS) or Pi stimulation, with or without BAY 11-7082; values are mean ± standard error of the mean (SEM; n = 4 independent isolations; *p ≤ 0.05 vs. vehicle control [Ctrl], #p ≤ 0.05 vs 20 μM BAY 11-7082 Ctrl). Dotted lines indicate corresponding median measurements from vehicle Ctrl. (D, E) qPCR analysis of primary hepatocytes shows Saa1 and Hp expression levels following LPS or Pi stimulation, with or without anti-IL1β, anti-IL6, or both antibodies in combination; values are mean ± standard error of the mean (SEM; n = 4 independent isolations; *p ≤ 0.05 vs. vehicle control [Ctrl], #p ≤ 0.05 vs. anti-IL1β Ctrl, $p ≤ 0.05 vs. anti-IL6 Ctrl, @p ≤ 0.05 vs. anti-IL1β + anti-IL6 Ctrl) where statistical analyses were calculated by two-way analysis of variance (ANOVA) followed by Tukey’s multiple comparison post hoc test. Dotted lines indicate corresponding median measurements from vehicle Ctrl.