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. 2022 Feb 11;11:e72872. doi: 10.7554/eLife.72872

Figure 3. Monopolar spindles break down upon dynein depletion.

Figure 3.

(A) Representative images of klp-18(RNAi) oocyte spindles (GFP::tubulin and GFP::histone) in germline counting and corresponding quantifications; dynein depletion leads to dissolution of monopoles, releasing chromosomes with associated microtubule bundles into the cytoplasm. Scale bars = 2.5 μm (B) Immunofluorescence (IF) imaging of monopole breakdown after dynein depletion; shown are tubulin (green), DNA (blue), ASPM-1 (red), and dynein (not shown in merge). Chromosomes released into the cytoplasm retain lateral microtubule associations (zooms). Scale bars = 2.5 μm; note that the +Auxin images are zoomed out more since chromosomes are more dispersed following monopole breakdown and a larger region needed to be imaged.