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. 2022 Feb 11;11:e72872. doi: 10.7554/eLife.72872

Figure 6. Microtubules reorganize into miniature bipolar spindles that can segregate chromosomes.

Figure 6.

(A) Immunofluorescence (IF) imaging of microtubules (green), DNA (blue), ASPM-1 (red), and dynein (not shown in merge) in monopolar spindle breakdown conditions (klp-18(RNAi) + short-term Dynein auxin-inducible degron [AID]). Microtubule bundles appear to reorganize around individual chromosomes, seen through ASPM-1 flanking either side of the chromosome; note that in these images ASPM-1 also appears to be on chromosomes, but that is background staining that sometimes occurs with this antibody (Wignall and Villeneuve, 2009) and is not real signal. (B) IF imaging of SPD-1 localization in the Dynein AID strain in control RNAi conditions (rows 1–4) or following klp-18(RNAi) (rows 5–7); shown are tubulin (green), DNA (blue), SPD-1 (red), and dynein (not shown in merge). SPD-1 does not localize to spindles in metaphase (rows 1, 3; 18/18 metaphases), but localizes to overlapping microtubules in anaphase spindles in the presence or absence of dynein (rows 2, 4; 17/17 anaphases). SPD-1 is not localized to monopolar spindles either before or after monopole breakdown (rows 5, 6; 60/60 monopoles and breakdowns), but can clearly be seen localized to miniature anaphases (row 7; 27/27 mini anaphases). Cortex is represented by the dashed line. All scale bars = 2.5 µm.