Video 11. Splaying of acentrosomal poles occurs in unarrested oocytes depleted of dynein.

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Another example of an unarrested oocyte expressing GFP::tubulin and GFP::histone, dissected into Meiosis Medium containing 100 μM auxin to deplete dynein. A spindle tries to form in the absence of dynein, leading to dynamic and unfocused poles. Anaphase segregation still occurs, albeit with a failure in spindle rotation. Corresponds to the movie in row 4 of Figure 2—figure supplement 3. Phenotypes are consistent across all oocytes filmed (n = 9). t = 0 is set to onset of meiosis II. Time elapsed shown in (min):(sec). Scale bar = 5 μm