Video 13. Monopolar spindles break down and individual chromosomes segregate following dynein depletion.

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Another example of a Dynein AID oocyte expressing GFP::tubulin and GFP::histone following klp-18(RNAi), dissected into Meiosis Medium containing 100 μM auxin to deplete dynein. In this example, reorganization of local microtubule bundles around individual chromosomes can be observed after breakdown of the monopolar spindle. Shortly after reorganization of these microtubules, anaphase-like segregation occurs synchronously across all chromosomes. Corresponds to Figure 4B. Phenotypes are consistent across all oocytes filmed (n = 5). Time elapsed shown in min:s. Scale bar = 5 μm.