Video 14. Dynamics of a normal monopolar spindle.

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Shows an oocyte expressing GFP::tubulin and GFP::histone following klp-18(RNAi), dissected into a Meiosis Medium control solution. Chromosomes extend outward towards microtubule plus ends forming a monopolar spindle during metaphase, but retract towards the minus ends during anaphase. Corresponds to Figure 4A. Phenotypes are consistent across all oocytes filmed (n = 6). Time elapsed shown in min:s. Scale bar = 5 μm.