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. 2022 Feb 11;11:e72872. doi: 10.7554/eLife.72872

Video 2. Dynein localizes to spindle poles in the Dynein AID strain in the absence of auxin.

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Shows a metaphase-arrested (emb-30(RNAi)) oocyte expressing mCherry::tubulin (top), dissected into control Meiosis Medium solution. DHC-1::degron::GFP (bottom) is clearly localized to spindle poles and faintly seen at kinetochores and spindle morphology does not change during the imaging timeframe in the absence of auxin. Corresponds to Figure 2C. Results are consistent across all oocytes filmed (n = 4). Time elapsed shown in min:s. Scale bar = 5 μm.