Video 7. Addition of auxin to metaphase-arrested oocytes in the TIR1 control strain does not perturb spindle architecture.

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Shows a metaphase-arrested oocyte expressing GFP::tubulin and GFP::histone in the TIR1 control strain (that contains TIR1 but lacks a degron tag on DHC-1). Dissection into Meiosis Medium containing 100 μM auxin yields no significant change in spindle length or organization, demonstrating that auxin addition itself is not responsible for phenotypes observed in our acute Dynein auxin-inducible degron (AID) imaging. Corresponds to Figure 2—figure supplement 2A. Phenotypes are consistent across all oocytes filmed (n = 5). Time elapsed shown in min:s. Scale bar = 5 μm.