Video 9. Normal oocytes undergo two rounds of bipolar spindle formation and anaphase segregation.

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Another example of an unarrested oocyte expressing GFP::tubulin and GFP::histone, dissected into control Meiosis Medium solution. After one round of segregation, a second bipolar spindle is formed, rotates perpendicular to the cortex, and successfully extrudes a second polar body. Corresponds to the movie in row 2 of Figure 2—figure supplement 3. Phenotypes are consistent across all oocytes filmed (n = 8). t = 0 is set to onset of meiosis II. Time elapsed shown in (min):(sec). Scale bar = 5 μm.