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. 2022 Mar 14;10:836747. doi: 10.3389/fbioe.2022.836747

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Copyright © 2022 Paterna, Rao, Adamo, Raccosta, Picciotto, Romancino, Noto, Touzet, Bongiovanni and Manno.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunoblot analysis of specific biomarkers (H+/ATPase and Alix) in Tetraselmis chuii cells lysate (Microalgae lysate; 10 µg) and nanoalgosomes isolated by TFF from Tetraselmis chuii fresh culture (R0), and after 1, 2, and 3 subculturing steps (R1, R2, and R3, respectively) (upper panel). Ponceau red staining is shown as loading control (bottom panel). Three independent experiments (n = 3) were performed.