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. 2022 Mar 14;13:837262. doi: 10.3389/fimmu.2022.837262

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Copyright © 2022 Liu, Qian, Zhou, Shen, Tan, Liu, Zhao, Shi, Qi, Zhou, Yang, Gao and Yang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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IL25 promoted the stemness of CRC cells. (A) The expression levels of CSC markers, namely, CD133 and LGR5, were examined in HT-29 and SW620 cells treated with recombinant IL25 in a concentration-dependent manner by RT-PCR. (B, C) The expression levels of CD133 and LGR5 were examined in HT-29 and SW620 cells treated with recombinant IL25 in a concentration and time-dependent manner by Western blotting. (D) Frequency (down) and representative day-7 images (up) of human colon adenomatous organoids treated with/without IL25 (50 ng/ml). (E) Sphere formation analysis of HT-29 and SW620 cells treated with recombinant IL25 in a concentration-dependent manner. Representative images (up) and the mean numbers and sphere size (down) of spheres are shown. Scale bar, 200 μm. Data present as mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001.