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. 2022 Mar 14;13:837262. doi: 10.3389/fimmu.2022.837262

Figure 4.

Figure 4

IL25 Deficiency Induced loss of CRC stemness. (A) Representative images of LGR5 IHC staining (left). Statistical analysis of LGR5 staining in WT and IL25KO tumors (right). (B) Stem cell markers CD133 and LGR5 expression were detected by multiplexed fluorescence staining in the WT and IL25KO AOM-DSS-induced CRC mouse models. The representative images show the expression of LGR5 (red), DAPI (blue), CD133 (green). (C) The expression levels of stem cells markers were examined in WT and IL25KO tumors by RT-PCR. (D) The expression levels of CSC markers, namely, DCLK1, ALDH1, and CD44, were examined in tumor tissues by Western blotting. (E) Western blotting of Stem cell markers levels in LoVo cells following IL25 silencing. (F) Frequency (right) and representative day-7 images (left) of adenomatous organoids from WT and IL25KO mice treated with/without IL25 (50 ng/ml). (G) Sphere formation analysis of LoVo cells following IL25 silencing. Representative images (left) and the mean numbers and sphere size (right) of spheres are shown. Data present as mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001.