Table 2.
Plasmids used in experiments.
| Name of plasmids | Details |
|---|---|
| pUC57-pcaspase-1 | Stored in our laboratory |
| pUC57-pGSDMD | Stored in our laboratory |
| pET-30a(+)-pcaspase-1 p20 | Prepared by this study, encoding 120-297aa of pcaspase-1 with a N-terminal 6×His tag |
| pET-30a(+)-His-pcaspase-1 | Prepared by this study, encoding full-length pcaspase-1 with a N-terminal 6×His tag |
| pET-30a(+)-pcaspase-1-His | Prepared by this study, encoding full-length pcaspase-1 with a C-terminal 6×His tag |
| pET-30a(+)-Δpcaspase-1-His | Prepared by this study, encoding pcaspase-1 without the 1-120aa prodomain and with a C-terminal 6×His tag |
| pcDNA3.1(+)-pcaspase-1 | Prepared by this study, encoding full-length pcaspase-1 |
| pEGFP-C1-pGSDMD | Prepared by this study, encoding full-length pGSDMD with a N-terminal fused enhanced green fluorescent protein |
| pET-30a(+)-His-pGSDMD D279A | Prepared by this study, encoding a pGSDMD mutant of which the aspartate (D)279 is replaced with an alanine (A) |
| pET-30a(+)-His-pGSDMD-CT | Prepared by this study, encoding 280-489aa of pGSDMD with a N-terminal 6×His tag |
| pcDNA3.1(+)-pGSDMD-CT | Prepared by this study, encoding 280-489aa of pGSDMD |
| pET-30a(+)-His-pGSDMD-NT | Prepared by our laboratory, encoding 1-279aa of pGSDMD with a N-terminal 6×His tag |
| pcDNA3.1(+)-pGSDMD-NT | Prepared by our laboratory, encoding 1-279aa of pGSDMD |
| pET-30a(+)-His-pGSDMD | Prepared by our laboratory, encoding full-length pGSDMD with a N-terminal 6×His tag |
| pcDNA3.1(+)-pGSDMD | Prepared by our laboratory, encoding full-length pGSDMD |