Skip to main content
. 2022 Mar 14;10:847908. doi: 10.3389/fcell.2022.847908

FIGURE 2.

FIGURE 2

(A) Basal body organization in Paramecium tetraurelia. Basal bodies (BB) are decorated by monoclonal anti-glutamylated tubulin ID5 antibodies (green). Note that in the anterior part of the cell (area 1 corresponding to the invariant field, traced in white), basal bodies are organized in doublets. In the posterior part of the cell, singlet basal bodies are observed (area 3). In the rest of the cell, doublets or singlets of basal bodies are observed (mixed field, area 2). Scale bar: 10 µm. (B,C) Immunofluorescence showing basal bodies decorated by ID5 antibodies (green) and cilia stained by anti-polyglutamylated tubulin (red). Transverse section showing that in the invariant field (area 1), each BB, which are organized in doublets, grow a cilium. Scale bar: 1 µm. (D) Schematic representation of the different steps (A–E) of BB duplication. (E) Longitudinal section of a ciliated basal body observed by transmission electron microscopy Scale bar: 200 nm. The arrows indicated the position of the three layers of the TZ. Terminal plate (T), intermediate plate (I) and axosomal plate (A). The dotted lines indicate the position of the transition fibers (1) and Y-links (2). (F) Schema of the BB appendages in a 2BB unit. The striated rootlet (SR) courses toward the anterior of the cell while the post-ciliary rootlet (Pc) is located near the 8th and 9th microtubule triplets. Also shown are the transverse rootlets on the anterior BB (Ta) and the posterior BB (Tp). (G) 3D representation showing the topographical relationships of BB and its anterior appendages: the striated ciliary rootlet (SR), the transverse microtubules (T) and the anterior left filament (ALF). The new BB (green disc). Reproduced/adapted with permission Aubusson et al, 2012. Journal of cell science, 125, 4395-4404 from Jerka-Diadosz et al., 2013.